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Leishmaniasis

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21 Table 2. Leishmaniasis Reference Diagnostic Laboratories in North America Laboratory a Testing available Submitting samples b Point of Contact McGill University, Montreal, CANADA • Culture • PCR (conventional and real time) • Species determination (DNA sequencing, DNA probes) • Antibody detection (DAT, rK39, ELISA Crude Antigen) Shipment instructions provided on request. Shipment preferred using McGill transport medium. In most cases, specimens should be sent to the relevant provincial public health laboratory, which will forward samples as appropriate. Momar Ndao, DVM, MSc, PhD National Reference Centre for Parasitolog y Research Institute of the McGill University Health Centre Room E03 5375 1001 Décarie Blvd Montreal, QC H4A 3J1 Tel: 514-934-8347 Fax: 514-934-8261 Email: momar.ndao@mcgill.ca https://www.mcgill.ca/ tropmed/nrcp Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA • Microscopic evaluation • Culture • PCR (conventional and real time) • Species determination (DNA sequencing analysis; also cellulose acetate electrophoresis) • Antibody detection (rK39 Rapid Test) Shipment instructions provided on request. Shipment preferred using CDC transport medium. Marcos E. de Almeida, PhD Centers for Disease Control and Prevention Division of Parasitic Diseases and Malaria 1600 Clion Road NE, Mailstop D-64 Building 23, 9th Floor, Room 439 Atlanta, GA 30329-4027 Tel: 404-718-4175/718-4126 Fax: 404-718-4191 Email: bnz0@cdc.gov http://www.cdc.gov/ parasites/leishmaniasis/ health_professionals Walter Reed Army Institute of Research (WRAIR), USA • Microscopic evaluation • Culture • PCR (real time) • Species determination (Cellulose acetate electrophoresis) • Xenodiagnosis (mice and hamsters) • Antibody detection (rK39) Shipment instructions provided on request. Shipment preferred using WRAIR transport medium. Services restricted to samples from U.S. military beneficiaries and DoD civilian workers. Sheila A. Peel, MSPH, PhD Leishmania Diagnostic Laboratory Walter Reed Army Institute of Research 503 Robert Grant Avenue Silver Spring, MD 20910-7500 Tel: 240-595-7353 Email: usarmy.detrick. medcom-wrair.mbx. leishmania-diagnostic@ mail.mil http://www.wrair.army.mil/ OtherServices_LDL.aspx Note: Please visit http://apps.who.int/whocc/List.aspx?cc_subject=Leishmaniasis& to access additional laboratories that are World Health Organization (WHO) Collaborating Centers, http://www.who.int/leishmaniasis/collaborating_centres/en/ a Additional WHO leishmaniasis laboratories are listed in WHO Technical Report Series 949 "Control of the leishmaniasis" pages 162–3 — whqlibdoc.who.int/trs/WHO_TRS_949_eng.pdf. b Recommend contact with reference laboratories in advance for instructions to optimize specimen collection and shipping. Tests performed in the above laboratories are provided free of charge.

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